Terminator

Part:BBa_K5507001:Design

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


rrnB-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 85


Design Notes

rrnB-T provides reliable and strong termination of transcription, minimizing read-through effects. It is well-suited for use in various bacterial expression systems. Furthermore, the hairpin structure formed by the terminator contributes to the stability of the mRNA, promoting better translation efficiency.



Source

rrnB-T amplified from pKK223-3 plasmid, and derived from the ribosomal RNA operon of E. coli.

References